首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1808篇
  免费   100篇
  国内免费   3篇
林业   133篇
农学   63篇
基础科学   4篇
  473篇
综合类   144篇
农作物   114篇
水产渔业   97篇
畜牧兽医   717篇
园艺   48篇
植物保护   118篇
  2023年   27篇
  2022年   25篇
  2021年   83篇
  2020年   83篇
  2019年   96篇
  2018年   76篇
  2017年   65篇
  2016年   73篇
  2015年   61篇
  2014年   67篇
  2013年   121篇
  2012年   140篇
  2011年   150篇
  2010年   86篇
  2009年   55篇
  2008年   115篇
  2007年   105篇
  2006年   97篇
  2005年   81篇
  2004年   64篇
  2003年   51篇
  2002年   46篇
  2001年   15篇
  2000年   12篇
  1999年   12篇
  1998年   6篇
  1997年   4篇
  1996年   8篇
  1995年   5篇
  1994年   6篇
  1993年   6篇
  1992年   4篇
  1991年   4篇
  1990年   7篇
  1989年   2篇
  1987年   2篇
  1986年   5篇
  1985年   6篇
  1984年   6篇
  1983年   5篇
  1982年   3篇
  1980年   4篇
  1979年   2篇
  1977年   2篇
  1974年   2篇
  1973年   2篇
  1972年   3篇
  1967年   2篇
  1958年   1篇
  1955年   1篇
排序方式: 共有1911条查询结果,搜索用时 15 毫秒
81.
82.
Plant Foods for Human Nutrition - Silver linden (Tilia tomentosa Moench, TtM) flowers possess several health-promoting properties, especially at the neurological level, such as intestinal...  相似文献   
83.
In previous studies we demonstrated that green and roasted coffee contains low molecular weight (LMW) compounds capable of inhibiting the ability of Streptococcus mutans, the major causative agent of human dental caries, to adhere to hydroxyapatite (HA) beads. This study addressed the ability of the whole high molecular weight coffee fraction (cHMW) and of its melanoidin and non-melanoidin components (GFC1-5), applied at concentrations that occur in coffee beverages, to (i) inhibit S. mutans growth; (ii) affect S. mutans sucrose-dependent adhesion to and detachment from saliva-coated HA beads (sHA); and (iii) inhibit biofilm development on microtiter plates. The results indicated that only cHMW is endowed with antimicrobial activity. The cHMW fraction and each of the five GFC components inhibited S. mutans adhesion, the strongest effect being exerted by cHMW (91%) and GFC1 (88%). S. mutans detachment from sHA was four times greater (~20%) with cHMW and the GFC1 and GFC4 melanoidins than with controls. Finally, biofilm production by S. mutans was completely abolished by cHMW and was reduced by 20% by the melanoidin components GFC2 and GFC4 and by the non-melanoidin component GFC5 compared with controls. Altogether these findings show that coffee beverage contains both LMW compounds and HMW melanoidin and non-melanoidin components with a strong ability to interfere in vitro with the S. mutans traits relevant for cariogenesis.  相似文献   
84.
Complexes of β-cyclodextrin (β-CD) and caffeine (Caf) with biophenols present in olive and olive oil (tyrosol, hydroxytyrosol, homovanillic acid, 3,4-dihydroxyphenylacetic acid, and protocatechuic acid) were investigated by NMR spectroscopy and thermodynamical-molecular dynamic studies to verify the formation of supermolecular aggregates. The obtained results indicated that the investigated biophenols form inclusion complexes with β-CD in a molar ratio of 1:1 in aqueous solution having binding constant values from 10- to 40-fold bigger than those of the corresponding complexes with Caf. Then, β-CD preferentially encloses the biophenol molecule, decreasing its bitter taste and, at the same time, preserving it against chemical and physical decomposition reactions that occur during storage.  相似文献   
85.
In order to observe the spatial phosphorus (P) fractions transformations in sediments in relation to bacterial abundance and enzyme hydrolysing organic P-alkaline phosphatase (APA), samples from 35 stations from eutrophic Sulejow Reservoir were taken in spring after flood and in summer after cyanobacterial bloom breakdown. The results show pronounced fluctuations: decrease of average total P in sediments, despite organic matter delivery after cyanobacterial bloom, in parallel with increase of labile P (8.3%) and Ca-bounded P (16.6%) fractions and decline of organic P fraction (28.5%). Higher alkaline activity in sediments in the spring delivered nutrients to water column and supported cyanobacterial bloom development during the summer. Positive correlation between APA and organic P (r?=?0.37, p?<?0.01, n?=?70) and negative with labile inorganic P (r?=??0.44, p?<?0.01, n?=?70) in sediments proved significant role of the APA in phosphorus transformation in sediments and internal loading in the reservoir. During summer, APA was significantly related to bacterial number (r?=?0.36, p?<?0.01, n?=?35) and bacterial abundance was correlated to organic matter content (r?=?0.36, p?<?0.01, n?=?35). Such pattern of temporal variations of P transformation in sediments indicates order of solutions for enhancement of recultivation effects of eutrophic dam reservoirs: (1) reduction of organic matter supply in spring and (2) sediment inactivation during summer.  相似文献   
86.
The sour cherry is known to exhibit the phenomenon of gametophytic self-incompatibility which prevents self-fertilization. In sour cherry, besides self-incompatible cultivars, there often occur self-compatible cultivars. This is due to the occurrence of natural mutations of the S-RNase or SFB genes and, consequently, loss of functionality of S-alleles. Here we present the results of the identification of S-haplotypes of 21 cultivars of sour cherry from various regions of Europe. The analyses were performed using methods based on the amplification of intron I and intron II of the S-RNase gene and fragments specific to the individual alleles of the S-RNase or SFB genes. The tested cultivars were found to contain 15 S-haplotypes: S1, S1?, S4, S6, S6m, S6m2, S9, S12, S13, S13?, S26, S35, S36a, S36b, and S36b2. The most frequently occurring S-haplotypes were S13? (61.9%), S36a (57.1%), and S26 (47.6%). On the basis of the results, 17 of the 21 cultivars were deduced to be self-compatible. The results will be of use in the production of sour cherry fruit by facilitating the selection of suitable pollinating cultivars. The results are also expected to be useful in the breeding of new cultivars of sour cherry when selecting genotypes for crosses.  相似文献   
87.
88.
89.
New aspects of soybean somatic embryogenesis   总被引:4,自引:0,他引:4  
Somatic embryo formation from immature cotyledons was improved in the following ways: by cutting into sections, supplementing culture media with spermine and using solid/liquid/solid type of culture. Cut cotyledons of the eight genotypes examined expressed a higher ability for somatic embryogenesis than whole cotyledons. Of the three polyamines tested, spermine considerably stimulated and putrescine slightly inhibited induction of somatic embryos. The ability of embryoid formation on medium with spermidine depended on the genotype. The solid/liquid/solid type of culture was better than the continuous solid culture. The best nitrogen ion content for the subculture of somatic embryos was 10 mM NH4NO3 and 30 mM KNO3. The possibility of using these modifications in Agrobacterium transformation is discussed.  相似文献   
90.
Summary The capacity of the maize genotype 4c1 to regenerate microcalli and embryos from cultured microspores has been examined by comparing various cold pretreatments and culture media, using microspores and pollen at different stages of development. Viability of cultured cells was tested with FDA and their development was traced with light and fluorescence microscopy using DAPI as a nuclear dye.It was found that a pre-incubation of dissected flowers floating in a liquid nutrient medium at 8°C during 10–14 days was most successful for the induction of cell division. Among the developmental stages tested only the microspores appeared to regenerate. Subculture at 25°C in the same liquid medium, supplemented with 0.1 mg/l TIBA, gave highest rates of microspore division, i.e. up to 70% at 4 to 6 days of culture.All pathways described earlier for maize androgenic embryogenesis were observed within the 4c1 genotype. Symmetric divisions occurred in cultured microspores but most frequently asymmetric divisions lead to the formation of microcalli within 12 days of culture. In at least 60% of all dividing microspores cells were derived from the generative nucleus. Microcalli further developed either into loose or compact calli. Compact calli formed embryo-like structures.Abbreviations DAPI 4,6-diamidino-2-phenylindole - Dicamba 3,6-dichloro-2-methoxy benzoic acid - 2,4D 2,4 dichlorophenoxyacetic acid - FDA fluorescein diacetate - PAA phenylacetic acid - TIBA 2,3,5-triiodobenzoic acid - YP medium Yu-Pei basal salt medium  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号